Unlike Windows 8, 10, or 11, Windows 7 has older USB handling and security models. The main causes include:
This removes all locks and partitions:
If you are seeing "Rufus access to device denied" on your Windows 7 machine, This article will walk you through every possible cause and solution, from quick two-minute fixes to advanced registry tweaks. rufus access to device denied windows 7
mountvol -N